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Paste in X, Y values:

Paste in the X and Y values here. Each X value must be a substrate concentration and it should be followed by the corresponding experimental measurement of the reaction rate, Y, with an intervening space or text separator. Usually there would be one X and Y value per line (e.g. if pasting from a spreadsheet) although the X and Y pairs can be entered in one line, if so desired. Duplicate measurements can be entered all together in the same manner as the corresponding pairs of X and Y values e.g. one X, Y pair per line. Data can be entered as ordinary decimal numbers, integers, exponentials ('E' or 'e' notation) or a mixture of these formats. Non-numerical text should not matter since the program will try to remove it. If the plot does not appear or the curve does not fit well please check that the data are entered correctly. Sometimes to get the fitting routine to work you need to scale down the X-values, e.g. by converting them all from mM to M or from uM to mM or nM to uM, etc. It definitely is worth spending time rescaling all the values on an axis if the fitting routine does not work initially. Note that the fitting relies on the measurement at the highest value of X providing a reasonable first estimate of Vmax. Tick the check-box below and click submit again if you suspect the enzyme shows co-operativity so that the allosteric parameters can be calculated!

Tick here and re-submit if curve looks sigmoid.